Recombinant:Article Title: Histone Deacetylase 11 Is an ε-N-Myristoyllysine Hydrolase.
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REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins HDAC1 (full length with C-terminal His-tag, C-terminal FLAG-tag, R65% purity) BPS Bioscience Cat# 50051 Lot # 170150-2 HDAC2 (full length with C-terminal His-tag, R88% purity) BPS Bioscience Cat# 50002 Lot # 160701 HDAC3/NcoR2 (full length with C-terminal His-tag, R80% purity) BPS Bioscience Cat# 50003 Lot # 130819 HDAC4 (a.a 627–1084 with N-terminal GST-tag, C-terminal His-tag, R89% purity) BPS Bioscience Cat# 50004 Lot # 130828-G HDAC5 (a.a. 656–1122 with C-terminal His-tag, R90% purity) BPS Bioscience Cat# 50005 Lot # 140211 HDAC6 (full length with C-terminal FLAG-tag, R88% purity) BPS Bioscience Cat# 50056 Lot # 151130-C HDAC7 (a.a. 383–end with N-terminal His-tag, >90% purity) Millipore Cat# 14-832 Lot # DAM1684803 HDAC8 (full length with C-terminal His-tag, R90% purity) BPS Bioscience Cat# 50008 Lot # 161216 HDAC9 (a.a. 604–1066 with C-terminal His-tag, R76% purity) BPS Bioscience Cat# 50009 Lot # 130502-20 HDAC10 (a.a. 2–631 with N-terminal FLAG-tag, R52% purity) BPS Bioscience Cat# 50060 Lot # 170111 HDAC11 (full length with N-terminal GST-tag, R20% purity) BPS Bioscience Cat# 50011 Lot # 151026-B HDAC11 (full length untagged, R71% purity) BPS Bioscience Cat# 50021 Lot # 160224 Trypsin (TPCK Treated, essentially salt-free, lyophilized powder, R10,000 BAEE units/mg protein) Sigma-Aldrich Cat# T1426 Bovine Serum Albumin (heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, R98%) Sigma-Aldrich Cat# A7030 Bovine Serum Albumin (heat shock fraction, protease free, essentially globulin free, pH 7, R98%) Sigma-Aldrich Cat# A3059 Bicinchoninic Acid Kit for Protein Determination Sigma-Aldrich Cat# BCA1 NuPAGE 4-12% Bis-Tris Protein Gels, 1.0 mm, 12-well ThermoFisher Scientific Cat# NP0322BOX NuPAGE LDS Sample Buffer (4X) ThermoFisher Scientific Cat# NP0007 NuPAGE Sample Reducing Agent (10X) ThermoFisher Scientific Cat# NP0009 NuPAGE MES SDS Running Buffer (20X) ThermoFisher Scientific Cat# NP0002 SimplyBlue SafeStain ThermoFisher Scientific Cat# LC6065 1a) Ac-Leu-Gly-Lys(ac)-AMC (Wegener et al., 2003; Madsen and Olsen, 2012b) N/A 1b) Ac-Leu-Gly-Lys(tfa)-AMC (Bradner et al., 2010; Madsen and Olsen, 2012b) N/A 1f) Ac-Leu-Gly-Lys(i-but)-AMC This work N/A 1g) Ac-Leu-Gly-Lys(i-val)-AMC This work N/A 1i) Ac-Leu-Gly-Lys(cr)-AMC (Madsen and Olsen, 2012b) N/A 1r) Ac-Leu-Gly-Lys(glu)-AMC (Anderson et al., 2017) N/A 1s) Ac-Leu-Gly-Lys(hmg)-AMC (Anderson et al., 2017) N/A 1t) Ac-Leu-Gly-Lys(bio)-AMC This work N/A 2a) Ac-Arg-His-Lys-Lys(ac)-AMC Fluor de lys-SIRT1 substrate (Enzo) Cat# KI177 3a) Ac-Arg-His-Lys(ac)-Lys(ac)-AMC Fluor de lys-HDAC8 substrate (Enzo) Cat# KI178 (Continued on next page) Cell Chemical Biology 25, 1–8.e1–e8, July 19, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 4a) Ac-Thr-Ala-Arg-Lys(ac)-AMC (Madsen and Olsen, 2012b) N/A 4d) Ac-Thr-Ala-Arg-Lys(dec)-AMC (Madsen et al., 2016) N/A 4e) Ac-Thr-Ala-Arg-Lys(myr)-AMC (Madsen et al., 2016) N/A 4x) Ac-Thr-Ala-Arg-Lys(lau)-AMC (Madsen et al., 2016) N/A 5a) Ac-Gln-Pro-Lys-Lys(ac)-AMC Fluor de lys-SIRT2 substrate (Enzo) and (Madsen et al., 2016) Cat# KI179 5c) Ac-Gln-Pro-Lys-Lys(hex)-AMC (Madsen et al., 2016) N/A 5d) Ac-Gln-Pro-Lys-Lys(dec)-AMC (Madsen et al., 2016) N/A 5e) Ac-Gln-Pro-Lys-Lys(myr)-AMC (Madsen et al., 2016) N/A 5f) Ac-Gln-Pro-Lys-Lys(i-but)-AMC (Madsen et al., 2016) N/A 5g) Ac-Gln-Pro-Lys-Lys(i-val)-AMC (Madsen et al., 2016) N/A 5i) Ac-Gln-Pro-Lys-Lys(cr)-AMC (Madsen et al., 2016) N/A 5u) Ac-Gln-Pro-Lys-Lys(but)-AMC (Madsen et al., 2016) N/A 5v) Ac-Gln-Pro-Lys-Lys(oct)-AMC (Madsen et al., 2016) N/A 5x) Ac-Gln-Pro-Lys-Lys(lau)-AMC (Madsen et al., 2016) N/A 5y) Ac-Gln-Pro-Lys-Lys(pal)-AMC (Madsen et al., 2016) N/A 6a) Ac-Glu-Thr-Asp-Lys(ac)-AMC (Galleano et al., 2016) N/A 6d) Ac-Glu-Thr-Asp-Lys(dec)-AMC (Galleano et al., 2016) N/A 6e) Ac-Glu-Thr-Asp-Lys(myr)-AMC (Galleano et al., 2016) N/A 6f) Ac-Glu-Thr-Asp-Lys(i-but)-AMC This work N/A 6g) Ac-Glu-Thr-Asp-Lys(i-val)-AMC (Galleano et al., 2016) N/A 6h) Ac-Glu-Thr-Asp-Lys(2-mebut)-AMC (Galleano et al., 2016) N/A 6i) Ac-Glu-Thr-Asp-Lys(cr)-AMC (Galleano et al., 2016) N/A 6j) Ac-Glu-Thr-Asp-Lys(meacr)-AMC This work N/A 6k) Ac-Glu-Thr-Asp-Lys(2-mecr)-AMC This work N/A 6l) Ac-Glu-Thr-Asp-Lys(3-mecr)-AMC This work N/A 6m) Ac-Glu-Thr-Asp-Lys(2E-dece)-AMC (Galleano et al., 2016) N/A 6n) Ac-Glu-Thr-Asp-Lys(3-hydroxybut)-AMC (Galleano et al., 2016) N/A 6o) Ac-Glu-Thr-Asp-Lys(3-hydroxydec)-AMC (Galleano et al., 2016) N/A 6p) Ac-Glu-Thr-Asp-Lys(acac)-AMC (Galleano et al., 2016) N/A 6q) Ac-Glu-Thr-Asp-Lys(3-oxodec)-AMC (Galleano et al., 2016) N/A 6x) Ac-Glu-Thr-Asp-Lys(lau)-AMC (Galleano et al., 2016) N/A 6z) Ac-Glu-Thr-Asp-Lys(2E-laue)-AMC (Galleano et al., 2016) N/A 6æ) Ac-Glu-Thr-Asp-Lys(3-hydroxylau)-AMC (Galleano et al., 2016) N/A 6ø) Ac-Glu-Thr-Asp-Lys(3-oxolau)-AMC (Galleano et al., 2016) N/A Trichostatin A Sigma-Aldrich Cat# T8552 SAHA (Vorinostat) Sigma-Aldrich Cat# SML0061 Trapoxin A Sigma-Aldrich Cat# T2580 Romidepsin Sigma-Aldrich Cat# SML1175 Entinostat Sigma-Aldrich Cat# EPS002 Apicidin A (Kitir et al., 2017) N/A ApiAAsuha (Kitir et al., 2017) N/A TpxBAsuha (Kitir et al., 2017) N/A TpxBAsu(Oct) (Kitir et al., 2017) N/A Software and Algorithms Prism 7.0 (for Mac and Windows) GraphPad https://www.graphpad.com/ scientific-software/prism/ e2 Cell Chemical Biology 25, 1–8.e1–e8, July 19, 2018
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